PrimePCR™ Probe Assay: EFR3B, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0030829
Assay Design:   Intron-spanning
Chromosome Location:   2:25344584-25351130question
Amplicon Length:   129
Splice Variants Targeted:   ENST00000401432 ENST00000403714 ENST00000402191 ENST00000405108 ENST00000264719 ENST00000545169

Gene Information

Description Not Available

Gene Symbol:   EFR3B
Gene Name:   EFR3 homolog B (S. cerevisiae)
Aliases:   KIAA0953
RefSeq:   NC_000002.11 NT_022184.15
Ensembl:   ENSG00000084710
Entrez:   22979
UniGene:   Hs.4892
Chromosome Mapping:   2p23.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.992600
y-intercept 35.150000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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