This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.
PrimePCR™ PreAmp for Probe Assay: COBRA1, Human
PrimePCR™ Template for Probe Assay: COBRA1, Human
NELFB is a subunit of negative elongation factor (NELF) which also includes NELFA (WHSC2; MIM 606026) either NELFC or NELFD (TH1L; MIM 605297) and NELFE (RDBP; MIM 154040). NELF acts with DRB sensitivity-inducing factor (DSIF) a heterodimer of SPT4 (SUPT4H1; MIM 603555) and SPT5 (SUPT5H; MIM 602102) to cause transcriptional pausing of RNA polymerase II (see MIM 180660) (Narita et al. 2003 [PubMed 12612062]).[supplied by OMIM Mar 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.