PrimePCR™ Probe Assay: ACOX1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0030792
Assay Design:   Intron-spanning
Chromosome Location:   17:73942848-73944480question
Amplicon Length:   148
Splice Variants Targeted:   ENST00000301608 ENST00000293217 ENST00000539791 ENST00000537812 ENST00000538781

Gene Information

The protein encoded by this gene is the first enzyme of the fatty acid beta-oxidation pathway which catalyzes the desaturation of acyl-CoAs to 2-trans-enoyl-CoAs. It donates electrons directly to molecular oxygen thereby producing hydrogen peroxide. Defects in this gene result in pseudoneonatal adrenoleukodystrophy a disease that is characterized by accumulation of very long chain fatty acids. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq Jul 2008]

Gene Symbol:   ACOX1
Gene Name:   acyl-CoA oxidase 1, palmitoyl
Aliases:   ACOX, MGC1198, PALMCOX, SCOX
RefSeq:   NC_000017.10 NT_010783.15 NG_008190.1
Ensembl:   ENSG00000161533
Entrez:   51
UniGene:   Hs.464137
Chromosome Mapping:   17q24-q25,17q25.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999000
y-intercept 35.020000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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