PrimePCR™ Probe Assay: CFHR5, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: CFHR5, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: CFHR5, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0030791
Assay Design:   Intron-spanning
Chromosome Location:   1:196971654-196973822question
Amplicon Length:   143
Splice Variants Targeted:   ENST00000256785 ENST00000367414

Gene Information

This gene is a member of a small complement factor H (CFH) gene cluster on chromosome 1. Each member of this gene family contains multiple short consensus repeats (SCRs) typical of regulators of complement activation. The protein encoded by this gene has nine SCRs with the first two repeats having heparin binding properties a region within repeats 5-7 having heparin binding and C reactive protein binding properties and the C-terminal repeats being similar to a complement component 3 b (C3b) binding domain. This protein co-localizes with C3 binds C3b in a dose-dependent manner and is recruited to tissues damaged by C-reactive protein. Allelic variations in this gene have been associated but not causally linked with two different forms of kidney disease: membranoproliferative glomerulonephritis type II (MPGNII) and hemolytic uraemic syndrome (HUS). [provided by RefSeq Jan 2010]

Gene Symbol:   CFHR5
Gene Name:   complement factor H-related 5
Aliases:   CFHL5, FHR-5, FHR5, FLJ10549, MGC133240
RefSeq:   NC_000001.10 NG_016365.1 NT_004487.19
Ensembl:   ENSG00000134389
Entrez:   81494
Chromosome Mapping:   1q31.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998900
y-intercept 35.330000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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