PrimePCR™ Probe Assay: GLRX2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
Your Price:   Log In
 

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0030784
Assay Design:   Intron-spanning
Chromosome Location:   1:193066841-193074655question
Amplicon Length:   92
Splice Variants Targeted:   ENST00000367439 ENST00000367440

Gene Information

The protein encoded by this gene is a member of the glutaredoxin family of proteins which maintain cellular thiol homeostasis. These proteins are thiol-disulfide oxidoreductases that use a glutathione-binding site and one or two active cysteines in their active site. This gene undergoes alternative splicing to produce multiple isoforms one of which is ubiquitously expressed and localizes to mitochondria where it functions in mitochondrial redox homeostasis and is important for the protection against and recovery from oxidative stress. Other isoforms which have more restrictive expression patterns show cytosolic and nuclear localization and are thought to function in cellular differentiation and transformation possibly with a role in tumor progression. [provided by RefSeq Aug 2011]

Gene Symbol:   GLRX2
Gene Name:   glutaredoxin 2
Aliases:   GRX2, bA101E13.1
RefSeq:   NC_000001.10 NT_004487.19
Ensembl:   ENSG00000023572
Entrez:   51022
UniGene:   Hs.458283
Chromosome Mapping:   1q31.2-q31.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.997400
y-intercept 35.580000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download