PrimePCR™ Probe Assay: TSEN54, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0030780
Assay Design:   Intron-spanning
Chromosome Location:   17:73515090-73517541question
Amplicon Length:   61
Splice Variants Targeted:   ENST00000333213 ENST00000545228 ENST00000434205

Gene Information

This gene encodes a subunit of the tRNA splicing endonuclease complex which catalyzes the removal of introns from precursor tRNAs. The complex is also implicated in pre-mRNA 3-prime end processing. Mutations in this gene result in pontocerebellar hypoplasia type 2.[provided by RefSeq Oct 2009]

Gene Symbol:   TSEN54
Gene Name:   tRNA splicing endonuclease 54 homolog (S. cerevisiae)
Aliases:   FLJ37147, PCH2A, PCH4, SEN54L, sen54
RefSeq:   NC_000017.10 NG_013041.1 NT_010783.15
Ensembl:   ENSG00000182173
Entrez:   283989
UniGene:   Hs.378501
Chromosome Mapping:   17q25.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 34.460000
Efficiency 107

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download