PrimePCR™ Probe Assay: PPIL6, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0030712
Assay Design:   Intron-spanning
Chromosome Location:   6:109740395-109748154question
Amplicon Length:   127
Splice Variants Targeted:   ENST00000440797 ENST00000521072 ENST00000520723 ENST00000417394 ENST00000518648 ENST00000424445

Gene Information

Description Not Available

Gene Symbol:   PPIL6
Gene Name:   peptidylprolyl isomerase (cyclophilin)-like 6
Aliases:   MGC27054, MGC27056, MGC41939, RSPH12, bA425D10.6, dJ919F19.1
RefSeq:   NC_000006.11 NT_025741.15
Ensembl:   ENSG00000185250
Entrez:   285755
UniGene:   Hs.32234
Chromosome Mapping:   6q21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.420000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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