PrimePCR™ Probe Assay: BFAR, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0030696
Assay Design:   Intron-spanning
Chromosome Location:   16:14758824-14761545question
Amplicon Length:   129
Splice Variants Targeted:   ENST00000261658 ENST00000426842

Gene Information

Description Not Available

Gene Symbol:   BFAR
Gene Name:   bifunctional apoptosis regulator
Aliases:   BAR, RNF47
RefSeq:   NC_000016.9 NT_010393.16
Ensembl:   ENSG00000103429
Entrez:   51283
Chromosome Mapping:   16p13.12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998800
y-intercept 35.010000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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