PrimePCR™ Probe Assay: ST14, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0030619
Assay Design:   Intron-spanning
Chromosome Location:   11:130064573-130066281question
Amplicon Length:   78
Splice Variants Targeted:   ENST00000278742 ENST00000525779

Gene Information

The protein encoded by this gene is an epithelial-derived integral membrane serine protease. This protease forms a complex with the Kunitz-type serine protease inhibitor HAI-1 and is found to be activated by sphingosine 1-phosphate. This protease has been shown to cleave and activate hepatocyte growth factor/scattering factor and urokinase plasminogen activator which suggest the function of this protease as an epithelial membrane activator for other proteases and latent growth factors. The expression of this protease has been associated with breast colon prostate and ovarian tumors which implicates its role in cancer invasion and metastasis. [provided by RefSeq Jul 2008]

Gene Symbol:   ST14
Gene Name:   suppression of tumorigenicity 14 (colon carcinoma)
Aliases:   HAI, MT-SP1, MTSP1, PRSS14, SNC19, TADG15, TMPRSS14
RefSeq:   NC_000011.9 NG_012132.1 NT_033899.8
Ensembl:   ENSG00000149418
Entrez:   6768
UniGene:   Hs.504315
Chromosome Mapping:   11q24-q25

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 34.630000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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