PrimePCR™ Probe Assay: WFDC9, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0030597
Assay Design:   Intron-spanning
Chromosome Location:   20:44237390-44238784question
Amplicon Length:   85
Splice Variants Targeted:   ENST00000326000

Gene Information

The WAP-type four-disulfide core (WFDC) domain or WAP signature motif contains eight cysteines forming four disulfide bonds at the core of the protein and functions as a protease inhibitor in many members of the WFDC domain family. This gene encodes a protein which contains a WFDC domain and is thus a member of the WFDC domain family. This gene and several other gene family members are clustered at 20q13.12. [provided by RefSeq Jul 2008]

Gene Symbol:   WFDC9
Gene Name:   WAP four-disulfide core domain 9
Aliases:   MGC126701, MGC126705, WAP9, dJ688G8.2
RefSeq:   NC_000020.10 NT_011362.10
Ensembl:   ENSG00000180205
Entrez:   259240
UniGene:   Hs.249828
Chromosome Mapping:   20q12-q13.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 34.720000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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