This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.
PrimePCR™ PreAmp for Probe Assay: WFDC9, Human
PrimePCR™ Template for Probe Assay: WFDC9, Human
The WAP-type four-disulfide core (WFDC) domain or WAP signature motif contains eight cysteines forming four disulfide bonds at the core of the protein and functions as a protease inhibitor in many members of the WFDC domain family. This gene encodes a protein which contains a WFDC domain and is thus a member of the WFDC domain family. This gene and several other gene family members are clustered at 20q13.12. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.