PrimePCR™ Probe Assay: SCGB1D2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0030585
Assay Design:   Intron-spanning
Chromosome Location:   11:62010899-62012249question
Amplicon Length:   128
Splice Variants Targeted:   ENST00000244926

Gene Information

The protein encoded by this gene is a member of the lipophilin subfamily part of the uteroglobin superfamily and is an ortholog of prostatein the major secretory glycoprotein of the rat ventral prostate gland. Lipophilin gene products are widely expressed in normal tissues especially in endocrine-responsive organs. Assuming that human lipophilins are the functional counterparts of prostatein they may be transcriptionally regulated by steroid hormones with the ability to bind androgens other steroids and possibly bind and concentrate estramustine a chemotherapeutic agent widely used for prostate cancer. Although the gene has been reported to be on chromosome 10 this sequence appears to be from a cluster of genes on chromosome 11 that includes mammaglobin 2. [provided by RefSeq Jul 2008]

Gene Symbol:   SCGB1D2
Gene Name:   secretoglobin, family 1D, member 2
Aliases:   LIPB, LPHB
RefSeq:   NC_000011.9 NT_167190.1
Ensembl:   ENSG00000124935
Entrez:   10647
UniGene:   Hs.204096
Chromosome Mapping:   11q13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 34.390000
Efficiency 103

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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