This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.
PrimePCR™ PreAmp for Probe Assay: SCGB1D2, Human
PrimePCR™ Template for Probe Assay: SCGB1D2, Human
The protein encoded by this gene is a member of the lipophilin subfamily part of the uteroglobin superfamily and is an ortholog of prostatein the major secretory glycoprotein of the rat ventral prostate gland. Lipophilin gene products are widely expressed in normal tissues especially in endocrine-responsive organs. Assuming that human lipophilins are the functional counterparts of prostatein they may be transcriptionally regulated by steroid hormones with the ability to bind androgens other steroids and possibly bind and concentrate estramustine a chemotherapeutic agent widely used for prostate cancer. Although the gene has been reported to be on chromosome 10 this sequence appears to be from a cluster of genes on chromosome 11 that includes mammaglobin 2. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.