PrimePCR™ Probe Assay: ARSG, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0030553
Assay Design:   Intron-spanning
Chromosome Location:   17:66397553-66416431question
Amplicon Length:   111
Splice Variants Targeted:   ENST00000452479 ENST00000448504

Gene Information

Sulfatases such as ARSG hydrolyze sulfate esters from sulfated steroids carbohydrates proteoglycans and glycolipids. They are involved in hormone biosynthesis modulation of cell signaling and degradation of macromolecules. This gene a novel arylsulfatase has activity toward pseudosubstrates including p-nitrocatechol sulfate and 4-methylumbelliferyl sulfate. Activity is competitively inhibited by phosphate. The unprocessed protein is active as a 63-kDa monomer and demonstrates an acidic pH optimum as typically seen for lysosomal sulfatases. The protein accumulates within lysosomes and is also a glycoprotein that binds specifically to mannose 6-phosphate receptors. [provided by RefSeq Jul 2008]

Gene Symbol:   ARSG
Gene Name:   arylsulfatase G
Aliases:   KIAA1001
RefSeq:   NC_000017.10 NT_010783.15
Ensembl:   ENSG00000141337
Entrez:   22901
UniGene:   Hs.437249
Chromosome Mapping:   17q24.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 34.540000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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