PrimePCR™ Probe Assay: RAB11FIP4, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: RAB11FIP4, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: RAB11FIP4, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0030512
Assay Design:   Intron-spanning
Chromosome Location:   17:29857355-29858676question
Amplicon Length:   146
Splice Variants Targeted:   ENST00000325874 ENST00000394744

Gene Information

Proteins of the large Rab GTPase family (see RAB1A; MIM 179508) have regulatory roles in the formation targeting and fusion of intracellular transport vesicles. RAB11FIP4 is one of many proteins that interact with and regulate Rab GTPases (Hales et al. 2001 [PubMed 11495908]).[supplied by OMIM Apr 2008]

Gene Symbol:   RAB11FIP4
Gene Name:   RAB11 family interacting protein 4 (class II)
Aliases:   FLJ00131, KIAA1821, MGC11316, MGC126566, RAB11-FIP4
RefSeq:   NC_000017.10 NT_010799.15
Ensembl:   ENSG00000131242
Entrez:   84440
Chromosome Mapping:   17q11.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998800
y-intercept 35.500000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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