PrimePCR™ Probe Assay: ZNF438, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0030470
Assay Design:   Intron-spanning
Chromosome Location:   10:31134394-31137530question
Amplicon Length:   150
Splice Variants Targeted:   ENST00000331737 ENST00000361310 ENST00000375311 ENST00000436087 ENST00000442986 ENST00000413025 ENST00000430896 ENST00000452305 ENST00000444692 ENST00000538351

Gene Information

Description Not Available

Gene Symbol:   ZNF438
Gene Name:   zinc finger protein 438
Aliases:   FLJ32761, MGC126671, bA330O11.1
RefSeq:   NC_000010.10 NT_008705.16
Ensembl:   ENSG00000183621
Entrez:   220929
UniGene:   Hs.660642
Chromosome Mapping:   10p11.23

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998600
y-intercept 35.800000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download