PrimePCR™ Probe Assay: MLYCD, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0030432
Assay Design:   Intron-spanning
Chromosome Location:   16:83945919-83948629question
Amplicon Length:   93
Splice Variants Targeted:   ENST00000262430

Gene Information

The product of this gene catalyzes the breakdown of malonyl-CoA to acetyl-CoA and carbon dioxide. Malonyl-CoA is an intermediate in fatty acid biosynthesis and also inhibits the transport of fatty acyl CoAs into mitochondria. Consequently the encoded protein acts to increase the rate of fatty acid oxidation. It is found in mitochondria peroxisomes and the cytoplasm. Mutations in this gene result in malonyl-CoA decarboyxlase deficiency. [provided by RefSeq Jul 2008]

Gene Symbol:   MLYCD
Gene Name:   malonyl-CoA decarboxylase
Aliases:   MCD, MGC59795
RefSeq:   NC_000016.9 NG_009079.1 NT_010498.15
Ensembl:   ENSG00000103150
Entrez:   23417
UniGene:   Hs.644610
Chromosome Mapping:   16q24

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 35.470000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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