PrimePCR™ Probe Assay: TIMP4, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0030394
Assay Design:   Intron-spanning
Chromosome Location:   3:12195870-12198344question
Amplicon Length:   77
Splice Variants Targeted:   ENST00000287814

Gene Information

This gene belongs to the TIMP gene family. The proteins encoded by this gene family are inhibitors of the matrix metalloproteinases a group of peptidases involved in degradation of the extracellular matrix. The secreted netrin domain-containing protein encoded by this gene is involved in regulation of platelet aggregation and recruitment and may play role in hormonal regulation and endometrial tissue remodeling. [provided by RefSeq Jul 2008]

Gene Symbol:   TIMP4
Gene Name:   TIMP metallopeptidase inhibitor 4
Aliases:   Not Available
RefSeq:   NC_000003.11 NG_011728.1 NT_022517.18
Ensembl:   ENSG00000157150
Entrez:   7079
UniGene:   Hs.591665
Chromosome Mapping:   3p25

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 34.700000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download