PrimePCR™ Probe Assay: UBE3A, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: UBE3A, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: UBE3A, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0030373
Assay Design:   Intron-spanning
Chromosome Location:   15:25605557-25615763question
Amplicon Length:   139
Splice Variants Targeted:   ENST00000232165 ENST00000356465 ENST00000397954 ENST00000438097 ENST00000428984

Gene Information

This gene encodes an E3 ubiquitin-protein ligase part of the ubiquitin protein degradation system. This imprinted gene is maternally expressed in brain and biallelically expressed in other tissues. Maternally inherited deletion of this gene causes Angelman Syndrome characterized by severe motor and intellectual retardation ataxia hypotonia epilepsy absence of speech and characteristic facies. The protein also interacts with the E6 protein of human papillomavirus types 16 and 18 resulting in ubiquitination and proteolysis of tumor protein p53. Alternative splicing of this gene results in three transcript variants encoding three isoforms with different N-termini. Additional transcript variants have been described but their full length nature has not been determined. [provided by RefSeq Jul 2008]

Gene Symbol:   UBE3A
Gene Name:   ubiquitin protein ligase E3A
Aliases:   ANCR, AS, E6-AP, EPVE6AP, FLJ26981, HPVE6A
RefSeq:   NC_000015.9 NG_002690.1 NT_026446.14 NG_009268.1
Ensembl:   ENSG00000114062
Entrez:   7337
Chromosome Mapping:   15q11.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.170000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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