PrimePCR™ Probe Assay: LYG2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0030364
Assay Design:   Intron-spanning
Chromosome Location:   2:99861855-99863181question
Amplicon Length:   76
Splice Variants Targeted:   ENST00000409238 ENST00000409679 ENST00000423306 ENST00000333017

Gene Information

The protein encoded by this gene contains a SLT domain a protein domain present in bacterial lytic transglycosylase (SLT) and in eukaryotic lysozymes (GEWL). SLT domain catalyzes the cleavage of the beta-14-glycosidic bond between N-acetylmuramic acid (MurNAc) and N-acetyglucosamine (GlcNAc). [provided by RefSeq Jul 2008]

Gene Symbol:   LYG2
Gene Name:   lysozyme G-like 2
Aliases:   LYGH, MGC119046, MGC119047, MGC119049
RefSeq:   NC_000002.11 NT_022171.15
Ensembl:   ENSG00000185674
Entrez:   254773
UniGene:   Hs.436468
Chromosome Mapping:   2q11.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 34.920000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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