PrimePCR™ Probe Assay: CSNK2A2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
Your Price:   Log In
 

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0030294
Assay Design:   Intron-spanning
Chromosome Location:   16:58192184-58198011question
Amplicon Length:   98
Splice Variants Targeted:   ENST00000262506

Gene Information

Description Not Available

Gene Symbol:   CSNK2A2
Gene Name:   casein kinase 2, alpha prime polypeptide
Aliases:   CK2A2, CSNK2A1, FLJ43934
RefSeq:   NC_000016.9 NT_010498.15
Ensembl:   ENSG00000070770
Entrez:   1459
UniGene:   Hs.82201
Chromosome Mapping:   16q21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 34.970000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download