PrimePCR™ Probe Assay: TMPRSS11A, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0030287
Assay Design:   Intron-spanning
Chromosome Location:   4:68780308-68784726question
Amplicon Length:   147
Splice Variants Targeted:   ENST00000508048 ENST00000334830 ENST00000513536 ENST00000396188

Gene Information

Description Not Available

Gene Symbol:   TMPRSS11A
Gene Name:   transmembrane protease, serine 11A
Aliases:   ECRG1, MGC133183
RefSeq:   NC_000004.11 NT_022778.16
Ensembl:   ENSG00000187054
Entrez:   339967
UniGene:   Hs.450110
Chromosome Mapping:   4q13.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998600
y-intercept 34.460000
Efficiency 103

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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