PrimePCR™ Probe Assay: GMEB2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0030275
Assay Design:   Intron-spanning
Chromosome Location:   20:62227070-62234342question
Amplicon Length:   149
Splice Variants Targeted:   ENST00000370069 ENST00000370077 ENST00000266068

Gene Information

This gene is a member of KDWK gene family. The product of this gene associates with GMEB1 protein and the complex is essential for parvovirus DNA replication. Study of rat homolog implicates the role of this gene in modulation of transactivation by the glucocorticoid receptor bound to glucocorticoid response elements. This gene appears to use multiple polyadenylation sites. [provided by RefSeq Jul 2008]

Gene Symbol:   GMEB2
Gene Name:   glucocorticoid modulatory element binding protein 2
Aliases:   KIAA1269, P79PIF, PIF79
RefSeq:   NC_000020.10 NT_011333.6
Ensembl:   ENSG00000101216
Entrez:   26205
UniGene:   Hs.473286
Chromosome Mapping:   20q13.33

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999000
y-intercept 34.460000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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