PrimePCR™ Probe Assay: DOK6, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0030260
Assay Design:   Intron-spanning
Chromosome Location:   18:67406236-67425051question
Amplicon Length:   134
Splice Variants Targeted:   ENST00000382713

Gene Information

DOK6 is a member of the DOK (see DOK1; MIM 602919) family of intracellular adaptors that play a role in the RET (MIM 164761) signaling cascade (Crowder et al. 2004 [PubMed 15286081]).[supplied by OMIM Mar 2008]

Gene Symbol:   DOK6
Gene Name:   docking protein 6
Aliases:   DOK5L, HsT3226, MGC20785
RefSeq:   NC_000018.9 NT_025028.14
Ensembl:   ENSG00000206052
Entrez:   220164
UniGene:   Hs.278285
Chromosome Mapping:   18q22.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 35.150000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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