PrimePCR™ Probe Assay: CCBE1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: CCBE1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: CCBE1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0030205
Assay Design:   Intron-spanning
Chromosome Location:   18:57134096-57136826question
Amplicon Length:   120
Splice Variants Targeted:   ENST00000439986 ENST00000398179

Gene Information

This gene is thought to function in extracellular matrix remodeling and migration. It is predominantly expressed in the ovary but down regulated in ovarian cancer cell lines and primary carcinomas suggesting its role as a tumour suppressor. Mutations in this gene have been associated with Hennekam lymphangiectasia-lymphedema syndrome a generalized lymphatic dysplasia in humans. [provided by RefSeq Mar 2010]

Gene Symbol:   CCBE1
Gene Name:   collagen and calcium binding EGF domains 1
Aliases:   FLJ30681, MGC50861
RefSeq:   NC_000018.9 NG_016990.1 NT_025028.14
Ensembl:   ENSG00000183287
Entrez:   147372
Chromosome Mapping:   18q21.32

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 34.480000
Efficiency 103

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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