PrimePCR™ Probe Assay: GNRH1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0030176
Assay Design:   Intron-spanning
Chromosome Location:   8:25276899-25279123question
Amplicon Length:   83
Splice Variants Targeted:   ENST00000421054 ENST00000276414

Gene Information

The protein encoded by this gene is secreted and then cleaved to form the 10 aa luteinizing hormone-releasing hormone (LHRH) also known as gonadoliberin-1 and prolactin release-inhibiting factor also known as GnRH-associated peptide 1. LHRH stimulates the release of luteinizing and follicle stimulating hormones which are important for reproduction. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq Jan 2010]

Gene Symbol:   GNRH1
Gene Name:   gonadotropin-releasing hormone 1 (luteinizing-releasing hormone)
Aliases:   GNRH, GRH, LHRH, LNRH
RefSeq:   NC_000008.10 NG_016457.1 NT_167187.1
Ensembl:   ENSG00000147437
Entrez:   2796
UniGene:   Hs.82963
Chromosome Mapping:   8p21-p11.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998600
y-intercept 34.500000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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