PrimePCR™ Probe Assay: GDF6, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0030151
Assay Design:   Intron-spanning
Chromosome Location:   8:97157687-97172582question
Amplicon Length:   104
Splice Variants Targeted:   ENST00000287020 ENST00000435084 ENST00000454970

Gene Information

This gene encodes a member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily of secreted signaling molecules. It is required for normal formation of some bones and joints in the limbs skull and axial skeleton. Mutations in this gene result in colobomata which are congenital abnormalities in ocular development and in Klippel-Feil syndrome (KFS) which is a congenital disorder of spinal segmentation. [provided by RefSeq Jul 2008]

Gene Symbol:   GDF6
Gene Name:   growth differentiation factor 6
Aliases:   BMP13, CDMP2, KFM, KFS, KFS1, KFSL, MCOP4, MCOPCB6, MGC158100, MGC158101, SCDO4, SGM1
RefSeq:   NC_000008.10 NG_008981.1 NT_008046.16
Ensembl:   ENSG00000156466
Entrez:   392255
UniGene:   Hs.492277
Chromosome Mapping:   8q22.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 34.620000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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