PrimePCR™ Probe Assay: STX1B, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: STX1B, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: STX1B, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0030127
Assay Design:   Intron-spanning
Chromosome Location:   16:31012497-31021731question
Amplicon Length:   107
Splice Variants Targeted:   ENST00000215095

Gene Information

Syntaxins are cellular receptors for transport vesicles (see MIM 603765). One of these proteins designated syntaxin 1B (STX1B) is directly implicated in the process of calcium-dependent synaptic transmission in rat brain (Smirnova et al. 1993 [PubMed 8105537]). The expression of this protein is transiently induced by long-term potentiation of synaptic responses in the rat hippocampus. The protein may play an important role in the excitatory pathway of synaptic transmission which is known to be implicated in several neurologic diseases.[supplied by OMIM Nov 2010]

Gene Symbol:   STX1B
Gene Name:   syntaxin 1B
Aliases:   STX1B1, STX1B2
RefSeq:   NC_000016.9 NT_010393.16
Ensembl:   ENSG00000099365
Entrez:   112755
Chromosome Mapping:   16p11.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 34.900000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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