PrimePCR™ Probe Assay: PRL, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0030013
Assay Design:   Intron-spanning
Chromosome Location:   6:22287753-22290512question
Amplicon Length:   150
Splice Variants Targeted:   ENST00000306482 ENST00000438606

Gene Information

This gene encodes the anterior pituitary hormone prolactin. This secreted hormone is a growth regulator for many tissues including cells of the immune system. It may also play a role in cell survival by suppressing apoptosis and it is essential for lactation. Alternative splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq Aug 2011]

Gene Symbol:   PRL
Gene Name:   prolactin
Aliases:   Not Available
RefSeq:   NC_000006.11 NT_007592.15
Ensembl:   ENSG00000172179
Entrez:   5617
UniGene:   Hs.1905
Chromosome Mapping:   6p22.2-p21.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 34.970000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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