PrimePCR™ Probe Assay: ZG16B, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
Your Price:   Log In
 

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0029986
Assay Design:   Intron-spanning
Chromosome Location:   16:2880714-2881873question
Amplicon Length:   131
Splice Variants Targeted:   ENST00000382280

Gene Information

Description Not Available

Gene Symbol:   ZG16B
Gene Name:   zymogen granule protein 16 homolog B (rat)
Aliases:   HRPE773, JCLN2, PAUF, PRO1567
RefSeq:   NC_000016.9 NT_010393.16
Ensembl:   ENSG00000162078
Entrez:   124220
UniGene:   Hs.105887
Chromosome Mapping:   16p13.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 34.490000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download