PrimePCR™ Probe Assay: ARHGAP44, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0029905
Assay Design:   Intron-spanning
Chromosome Location:   17:12860006-12862117question
Amplicon Length:   112
Splice Variants Targeted:   ENST00000340825 ENST00000379672 ENST00000538915 ENST00000544416 ENST00000262444

Gene Information

Description Not Available

Gene Symbol:   ARHGAP44
Gene Name:   Rho GTPase activating protein 44
Aliases:   KIAA0672, NPC-A-10, RICH2
RefSeq:   NC_000017.10 NG_015808.1 NT_010718.16
Ensembl:   ENSG00000006740
Entrez:   9912
UniGene:   Hs.499758
Chromosome Mapping:   17p12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 34.730000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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