PrimePCR™ Probe Assay: NUDT3, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0029750
Assay Design:   Intron-spanning
Chromosome Location:   6:34256602-34261281question
Amplicon Length:   131
Splice Variants Targeted:   ENST00000358797

Gene Information

NUDT3 belongs to the MutT or Nudix protein family. Nudix proteins act as homeostatic checkpoints at important stages in nucleoside phosphate metabolic pathways guarding against elevated levels of potentially dangerous intermediates like 8-oxo-dGTP which promotes AT-to-CG transversions (Safrany et al. 1998 [PubMed 9822604]).[supplied by OMIM Feb 2011]

Gene Symbol:   NUDT3
Gene Name:   nudix (nucleoside diphosphate linked moiety X)-type motif 3
Aliases:   DIPP, DIPP-1, DIPP1
RefSeq:   NC_000006.11 NT_007592.15
Ensembl:   ENSG00000112664
Entrez:   11165
UniGene:   Hs.188882
Chromosome Mapping:   6p21.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 34.730000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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