This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.
PrimePCR™ PreAmp for Probe Assay: NUDT3, Human
PrimePCR™ Template for Probe Assay: NUDT3, Human
NUDT3 belongs to the MutT or Nudix protein family. Nudix proteins act as homeostatic checkpoints at important stages in nucleoside phosphate metabolic pathways guarding against elevated levels of potentially dangerous intermediates like 8-oxo-dGTP which promotes AT-to-CG transversions (Safrany et al. 1998 [PubMed 9822604]).[supplied by OMIM Feb 2011]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.