PrimePCR™ Probe Assay: GRIK3, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0029747
Assay Design:   Intron-spanning
Chromosome Location:   1:37315916-37319255question
Amplicon Length:   120
Splice Variants Targeted:   ENST00000373091 ENST00000373093

Gene Information

Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. This gene product belongs to the kainate family of glutamate receptors which are composed of four subunits and function as ligand-activated ion channels. It is not certain if the subunit encoded by this gene is subject to RNA editing as the other 2 family members (GRIK1 and GRIK2). A Ser310Ala polymorphism has been associated with schizophrenia and there are conflicting reports of its association with the pathogenesis of delirium tremens in alcoholics. [provided by RefSeq Jul 2008]

Gene Symbol:   GRIK3
Gene Name:   glutamate receptor, ionotropic, kainate 3
Aliases:   EAA5, GLR7, GLUR7, GluR7a
RefSeq:   NC_000001.10 NG_011447.1 NT_032977.9
Ensembl:   ENSG00000163873
Entrez:   2899
UniGene:   Hs.128848
Chromosome Mapping:   1p34-p33

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998600
y-intercept 34.170000
Efficiency 103

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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