PrimePCR™ Probe Assay: SS18, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0029741
Assay Design:   Intron-spanning
Chromosome Location:   18:23619367-23632651question
Amplicon Length:   88
Splice Variants Targeted:   ENST00000415083 ENST00000269138 ENST00000269137 ENST00000542420 ENST00000542743 ENST00000545952 ENST00000539849 ENST00000539244

Gene Information

Description Not Available

Gene Symbol:   SS18
Gene Name:   synovial sarcoma translocation, chromosome 18
Aliases:   MGC116875, SSXT, SYT
RefSeq:   NC_000018.9 NT_010966.14
Ensembl:   ENSG00000141380
Entrez:   6760
UniGene:   Hs.404263
Chromosome Mapping:   18q11.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998300
y-intercept 34.420000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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