PrimePCR™ Probe Assay: AGBL1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0029715
Assay Design:   Intron-spanning
Chromosome Location:   15:86838618-86940693question
Amplicon Length:   89
Splice Variants Targeted:   ENST00000389298 ENST00000421325 ENST00000441037

Gene Information

Description Not Available

Gene Symbol:   AGBL1
Gene Name:   ATP/GTP binding protein-like 1
Aliases:   FLJ32310, MGC149239
RefSeq:   NC_000015.9 NT_010274.17
Ensembl:   ENSG00000166748
Entrez:   123624
UniGene:   Hs.679833
Chromosome Mapping:   15q25.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998700
y-intercept 34.700000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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