PrimePCR™ Probe Assay: URI1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0029714
Assay Design:   Intron-spanning
Chromosome Location:   19:30498497-30500005question
Amplicon Length:   113
Splice Variants Targeted:   ENST00000360605 ENST00000542441 ENST00000312051 ENST00000392271

Gene Information

Description Not Available

Gene Symbol:   URI1
Gene Name:   URI1, prefoldin-like chaperone
RefSeq:   NM_001252641 NR_045557 NM_003796
Ensembl:   ENSG00000105176
Entrez:   8725
UniGene:   Hs.466391
Chromosome Mapping:   chr19:30433145-30507519

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 35.040000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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