PrimePCR™ Probe Assay: RRP36, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: RRP36, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: RRP36, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0029701
Assay Design:   Intron-spanning
Chromosome Location:   6:42993781-42994972question
Amplicon Length:   126
Splice Variants Targeted:   ENST00000244496

Gene Information

RRP36 functions at an early stage in the processing of 35S preribosomal RNA into the mature 18S species (Gerus et al. 2010 [PubMed 20038530]).[supplied by OMIM Jul 2010]

Gene Symbol:   RRP36
Gene Name:   ribosomal RNA processing 36 homolog (S. cerevisiae)
Aliases:   C6orf153, dJ20C7.4
RefSeq:   NC_000006.11 NT_007592.15
Ensembl:   ENSG00000124541
Entrez:   88745
Chromosome Mapping:   6p21.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 34.970000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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