PrimePCR™ Probe Assay: ZNF16, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0029653
Assay Design:   Intron-spanning
Chromosome Location:   8:146157889-146171445question
Amplicon Length:   107
Splice Variants Targeted:   ENST00000276816 ENST00000394909 ENST00000532351 ENST00000527811

Gene Information

The protein encoded by this gene contains a C2H2 type of zinc finger and thus may function as a transcription factor. This gene is located in a region close to ZNF7/KOX4 a gene also encoding a zinc finger protein on chromosome 8. Two alternatively spliced variants encoding the same protein have been identified. [provided by RefSeq Jul 2008]

Gene Symbol:   ZNF16
Gene Name:   zinc finger protein 16
Aliases:   KOX9
RefSeq:   NC_000008.10 NT_037704.5
Ensembl:   ENSG00000170631
Entrez:   7564
UniGene:   Hs.493225
Chromosome Mapping:   8q24

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 34.990000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download