PrimePCR™ Probe Assay: NBEAL2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0029640
Assay Design:   Intron-spanning
Chromosome Location:   3:47041794-47042537question
Amplicon Length:   118
Splice Variants Targeted:   ENST00000450053 ENST00000416683 ENST00000292309 ENST00000383740

Gene Information

The protein encoded by this gene contains a beige and Chediak-Higashi (BEACH) domain and multiple WD40 domains and may play a role in megakaryocyte alpha-granule biogenesis. Mutations in this gene are a cause of gray platelet syndrome. [provided by RefSeq Dec 2011]

Gene Symbol:   NBEAL2
Gene Name:   neurobeachin-like 2
Aliases:   KIAA0540
RefSeq:   NC_000003.11 NT_022517.18
Ensembl:   ENSG00000160796
Entrez:   23218
UniGene:   Hs.437043
Chromosome Mapping:   3p21.31

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.997900
y-intercept 35.160000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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