PrimePCR™ Probe Assay: ARHGEF26, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0029629
Assay Design:   Intron-spanning
Chromosome Location:   3:153972579-153973222question
Amplicon Length:   108
Splice Variants Targeted:   ENST00000356448 ENST00000465093

Gene Information

This gene encodes a member of the Rho-guanine nucleotide exchange factor (Rho-GEF) family. These proteins regulate Rho GTPases by catalyzing the exchange of GDP for GTP. The encoded protein specifically activates RhoG and plays a role in the promotion of macropinocytosis. Underexpression of the encoded protein may be a predictive marker of chemoresistant disease. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq Oct 2011]

Gene Symbol:   ARHGEF26
Gene Name:   Rho guanine nucleotide exchange factor (GEF) 26
Aliases:   CSGEF, DKFZp434D146, HMFN1864, SGEF
RefSeq:   NC_000003.11 NT_005612.16
Ensembl:   ENSG00000114790
Entrez:   26084
UniGene:   Hs.240845
Chromosome Mapping:   3q25.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 34.990000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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