PrimePCR™ Probe Assay: SIM2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0029624
Assay Design:   Intron-spanning
Chromosome Location:   21:38095346-38098489question
Amplicon Length:   126
Splice Variants Targeted:   ENST00000290399 ENST00000431229 ENST00000430056

Gene Information

SIM1 and SIM2 genes are Drosophila single-minded (sim) gene homologs. The Drosophila sim gene encodes a transcription factor that is a master regulator of fruit fly neurogenesis. SIM2 maps within the so-called Down syndrome chromosomal region. Based on the mapping position its potential function as transcriptional repressor and similarity to Drosophila sim it is proposed that SIM2 may contribute to some specific Down syndrome phenotypes [provided by RefSeq Jul 2008]

Gene Symbol:   SIM2
Gene Name:   single-minded homolog 2 (Drosophila)
Aliases:   MGC119447, SIM, bHLHe15
RefSeq:   NC_000021.8 NG_016193.1 NT_011512.11
Ensembl:   ENSG00000159263
Entrez:   6493
UniGene:   Hs.146186
Chromosome Mapping:   21q22.2,21q22.13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.997500
y-intercept 36.640000
Efficiency 88

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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