PrimePCR™ Probe Assay: TTC21B, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0029602
Assay Design:   Intron-spanning
Chromosome Location:   2:166771899-166773895question
Amplicon Length:   150
Splice Variants Targeted:   ENST00000243344

Gene Information

This gene encodes a member of TTC21 family containing several tetratricopeptide repeat (TPR) domains. This protein is localized to the cilium axoneme and may play a role in retrograde intraflagellar transport in cilia. Mutations in this gene are associated with various ciliopathies nephronophthisis 12 and asphyxiating thoracic dystrophy 4. [provided by RefSeq Oct 2011]

Gene Symbol:   TTC21B
Gene Name:   tetratricopeptide repeat domain 21B
Aliases:   FLJ11457, THM1
RefSeq:   NC_000002.11 NT_005403.17
Ensembl:   ENSG00000123607
Entrez:   79809
UniGene:   Hs.310672
Chromosome Mapping:   2q24.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 36.020000
Efficiency 93

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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