This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.
PrimePCR™ PreAmp for Probe Assay: TTC21B, Human
PrimePCR™ Template for Probe Assay: TTC21B, Human
This gene encodes a member of TTC21 family containing several tetratricopeptide repeat (TPR) domains. This protein is localized to the cilium axoneme and may play a role in retrograde intraflagellar transport in cilia. Mutations in this gene are associated with various ciliopathies nephronophthisis 12 and asphyxiating thoracic dystrophy 4. [provided by RefSeq Oct 2011]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.