This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.
PrimePCR™ PreAmp for Probe Assay: AD000671.1, Human
PrimePCR™ Template for Probe Assay: AD000671.1, Human
This gene encodes a protein which contains multiple domains including a CXXC zinc finger three PHD zinc fingers two FY-rich domains and a SET (suppressor of variegation enhancer of zeste and trithorax) domain. The SET domain is a conserved C-terminal domain that characterizes proteins of the MLL (mixed-lineage leukemia) family. This gene is ubiquitously expressed in adult tissues. It is also amplified in solid tumor cell lines and may be involved in human cancer. Two alternatively spliced transcript variants encoding distinct isoforms have been reported for this gene however the full length nature of the shorter transcript is not known. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.