PrimePCR™ Probe Assay: ZFYVE26, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
Your Price:   Log In
 

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0029583
Assay Design:   Intron-spanning
Chromosome Location:   14:68256256-68257405question
Amplicon Length:   147
Splice Variants Targeted:   ENST00000347230 ENST00000555452 ENST00000411699

Gene Information

This gene encodes a protein which contains a FYVE zinc finger binding domain. The presence of this domain is thought to target these proteins to membrane lipids through interaction with phospholipids in the membrane. Mutations in this gene are associated with autosomal recessive spastic paraplegia-15. [provided by RefSeq Oct 2008]

Gene Symbol:   ZFYVE26
Gene Name:   zinc finger, FYVE domain containing 26
Aliases:   DKFZp686F19106, DKFZp781H1112, FYVE-CENT, KIAA0321, SPG15
RefSeq:   NG_023267.1 NC_000014.8 NG_011836.1 NT_026437.12
Ensembl:   ENSG00000072121
Entrez:   23503
UniGene:   Hs.98041
Chromosome Mapping:   14q24.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.480000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download