PrimePCR™ Probe Assay: ADAMTS7, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: ADAMTS7, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: ADAMTS7, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0029582
Assay Design:   Intron-spanning
Chromosome Location:   15:79069118-79069884question
Amplicon Length:   135
Splice Variants Targeted:   ENST00000388820

Gene Information

The protein encoded by this gene is a member of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs) family. Members of this family share several distinct protein modules including a propeptide region a metalloproteinase domain a disintegrin-like domain and a thrombospondin type 1 (TS) motif. Individual members of this family differ in the number of C-terminal TS motifs and some have unique C-terminal domains. The protein encoded by this gene contains two C-terminal TS motifs. [provided by RefSeq Jul 2008]

Gene Symbol:   ADAMTS7
Gene Name:   ADAM metallopeptidase with thrombospondin type 1 motif, 7
Aliases:   ADAM-TS 7, ADAM-TS7, ADAMTS-7, DKFZp434H204
RefSeq:   NC_000015.9 NG_011492.1 NT_010194.17
Ensembl:   ENSG00000136378
Entrez:   11173
Chromosome Mapping:   15q24.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 36.240000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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