PrimePCR™ Probe Assay: EFCAB11, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0029541
Assay Design:   Intron-spanning
Chromosome Location:   14:90398870-90420264question
Amplicon Length:   132
Splice Variants Targeted:   ENST00000316738 ENST00000555872 ENST00000556609 ENST00000538485 ENST00000556005 ENST00000267544

Gene Information

Description Not Available

Gene Symbol:   EFCAB11
Gene Name:   EF-hand calcium binding domain 11
Aliases:   C14orf143, DKFZp547I1415, FLJ51529
RefSeq:   NC_000014.8 NG_009164.1 NT_026437.12
Ensembl:   ENSG00000140025
Entrez:   90141
UniGene:   Hs.123232
Chromosome Mapping:   14q32.11

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 34.850000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download