PrimePCR™ Probe Assay: JUP, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0029495
Assay Design:   Intron-spanning
Chromosome Location:   17:39923775-39925373question
Amplicon Length:   181
Splice Variants Targeted:   ENST00000393930 ENST00000310706 ENST00000393931 ENST00000449889 ENST00000437187 ENST00000420370 ENST00000540235

Gene Information

This gene encodes a major cytoplasmic protein which is the only known constituent common to submembranous plaques of both desmosomes and intermediate junctions. This protein forms distinct complexes with cadherins and desmosomal cadherins and is a member of the catenin family since it contains a distinct repeating amino acid motif called the armadillo repeat. Mutation in this gene has been associated with Naxos disease. Alternative splicing occurs in this gene; however not all transcripts have been fully described. [provided by RefSeq Jul 2008]

Gene Symbol:   JUP
Gene Name:   junction plakoglobin
Aliases:   ARVD12, CTNNG, DP3, DPIII, PDGB, PKGB
RefSeq:   NC_000017.10 NG_009090.1 NT_010783.15
Ensembl:   ENSG00000173801
Entrez:   3728
UniGene:   Hs.514174
Chromosome Mapping:   17q21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.370000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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