PrimePCR™ Probe Assay: OPRM1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: OPRM1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: OPRM1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0029490
Assay Design:   Intron-spanning
Chromosome Location:   6:154411143-154412144question
Amplicon Length:   199
Splice Variants Targeted:   ENST00000434900 ENST00000520708 ENST00000518759 ENST00000330432 ENST00000428397 ENST00000452687 ENST00000229768 ENST00000419506 ENST00000524163 ENST00000414028 ENST00000435918 ENST00000337049 ENST00000522555 ENST00000522236 ENST00000360422

Gene Information

This gene encodes one of three opioid receptors. The mu opioid receptor is the principal target of endogenous opioid peptides and opioid analgesic agents. Mutations in this gene have been associated with opioid and alcohol addiction and variations in pain sensitivity. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Sep 2011]

Gene Symbol:   OPRM1
Gene Name:   opioid receptor, mu 1
Aliases:   KIAA0403, LMOR, MOR, MOR1, OPRM
RefSeq:   NC_000006.11 NT_025741.15 NG_021208.1
Ensembl:   ENSG00000112038
Entrez:   4988
Chromosome Mapping:   6q24-q25

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 35.770000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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