PrimePCR™ Probe Assay: C2orf78, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0029484
Assay Design:   Intron-spanning
Chromosome Location:   2:74011465-74040743question
Amplicon Length:   179
Splice Variants Targeted:   ENST00000409561 ENST00000342345

Gene Information

Description Not Available

Gene Symbol:   C2orf78
Gene Name:   chromosome 2 open reading frame 78
Aliases:   COG5373, FLJ43987, hCG1989538
RefSeq:   NC_000002.11 NT_022184.15
Ensembl:   ENSG00000187833
Entrez:   388960
UniGene:   Hs.456833
Chromosome Mapping:   2p13.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 34.860000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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