PrimePCR™ Probe Assay: PPIE, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0029482
Assay Design:   Intron-spanning
Chromosome Location:   1:40208901-40209547question
Amplicon Length:   91
Splice Variants Targeted:   ENST00000470018 ENST00000324379 ENST00000356511 ENST00000497370 ENST00000470213 ENST00000372830

Gene Information

The protein encoded by this gene is a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. This protein contains a highly conserved cyclophilin (CYP) domain as well as an RNA-binding domain. It was shown to possess PPIase and protein folding activities and it also exhibits RNA-binding activity. Alternative splicing results in multiple transcript variants. A related pseudogene which is also located on chromosome 1 has been identified. [provided by RefSeq Aug 2010]

Gene Symbol:   PPIE
Gene Name:   peptidylprolyl isomerase E (cyclophilin E)
Aliases:   CYP-33, CYP33, MGC111222, MGC3736
RefSeq:   NC_000001.10 NT_032977.9
Ensembl:   ENSG00000084072
Entrez:   10450
UniGene:   Hs.524690
Chromosome Mapping:   1p32

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 34.760000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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