PrimePCR™ Probe Assay: SALL4, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
Your Price:   Log In
 

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0029474
Assay Design:   Intron-spanning
Chromosome Location:   20:50401130-50405492question
Amplicon Length:   157
Splice Variants Targeted:   ENST00000217086 ENST00000395997 ENST00000371539

Gene Information

The protein encoded by this gene may be a zinc finger transcription factor. Defects in this gene are a cause of Duane-radial ray syndrome (DRRS). [provided by RefSeq Jul 2008]

Gene Symbol:   SALL4
Gene Name:   sal-like 4 (Drosophila)
Aliases:   DRRS, HSAL4, MGC133050, ZNF797, dJ1112F19.1
RefSeq:   NC_000020.10 NG_008000.1 NT_011362.10
Ensembl:   ENSG00000101115
Entrez:   57167
UniGene:   Hs.517113
Chromosome Mapping:   20q13.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.220000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download