PrimePCR™ Probe Assay: XG, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0029466
Assay Design:   Intron-spanning
Chromosome Location:   X:2715353-2729429question
Amplicon Length:   108
Splice Variants Targeted:   ENST00000381174 ENST00000419513 ENST00000509484 ENST00000426774 ENST00000533923

Gene Information

This gene encodes the XG blood group antigen and is located at the pseudoautosomal boundary on the short (p) arm of chromosome X. The three 5' exons reside in the pseudoautosomal region and the remaining exons within the X-specific end. A truncated copy of this gene is found on the Y chromosome at the pseudoautosomal boundary. It is transcribed but not expected to make a Y-chromosome specific gene product. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Nov 2008]

Gene Symbol:   XG
Gene Name:   Xg blood group
Aliases:   MGC118758, MGC118759, MGC118760, MGC118761, PBDX
RefSeq:   NC_000023.10 NT_167197.1 NG_011627.1
Ensembl:   ENSG00000124343
Entrez:   7499
UniGene:   Hs.179675
Chromosome Mapping:   Xp22.33

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 35.310000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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